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DPPH
DPPH Assay
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DPPH Assay
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8:15
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DPPH Radical Scavenging Method-Total Antioxidant Capacity Assessment
Principle: DPPH(1,1-Diphenyl-2-picrylhydrazyl) is a stable free radial with red color(absorbed at 517nm). If free radials have been scavenged, DPPH will generated it's color to yellow. This assay uses this character to show free radical scavenging activity.
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Ensayo antioxidante DPPH La metodología DPPH(2,2,1-diphenyl-1-picrylhydrazyl) fue desarrollada por Brand-Williams y hace uso del DPPH de radical libre estable, que tiene un color púrpura fuerte que se puede medir espectrofotométricamente(517 nm). En presencia de compuestos que son capaces de transferir un electrón o donar hidrógeno, el DPPH se decolorará hacía un amarillo. Fuentes: [1]https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1618-6 [2]https://www.sciencedirect.com/scienc
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